alpha smooth muscle Actin Antibody
Source: Monoclonal Antibodies Catalog No. : CY1132
alpha smooth muscle Actin Antibody (CY1132)
UniProt:P62736
Application:WB,IHC,ICC/IF,FC
Reactivity:Human,Mouse,Rat
Source:Rabbit mAb
References（7）
http://www.abways.com/showproduct.asp?cid=CY1132
Data Sheet
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UniProt
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Mol Weight42kDa
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Alternative NamesActin, aortic smooth muscle, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, ACTA2, ACTSA, ACTVS, GIG46
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ImmunogenA synthesized peptide derived from human alpha smooth muscle Actin
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionInvolved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC,ICC/IF,FC
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Recommended dilutionWB 1:1000~1:10000 IHC 1:500~1:1000 ICC/IF 1:100~1:500 FC 1:20
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All lanes use the Antibody at 1:3W dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:3W dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:3W dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:3W dilution for 1 hour at room temperature. -
Western blot analysis of alpha smooth muscle Actin expression in (1)A549 cell lysate; (2)C2C12 cell lysate using alpha-SMA antibody. -
Immunohistochemical analysis of paraffin-embedded human colon, using alpha smooth muscle Actin Antibody. -
Immunofluorescent analysis of A431 cells, using alpha smooth muscle Actin Antibody . -
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature. -
Immunofluorescent analysis using the Antibody at 1:50 dilution. -
Immunofluorescent analysis using the Antibody at 1:150 dilution. -
Immunofluorescent analysis using the Antibody at 1:500 dilution.
References （7）
Contribution of acid sphingomyelinase to angiotensin II-induced vascular adventitial remodeling via membrane rafts/Nox2 signal pathway.-Life Sciences
Mesenchymal stem cells maintain the stemness of colon cancer stem cells via interleukin-8/mitogen-activated protein kinase signaling pathway-Experimental Biology and Medicine (EXP BIOL MED)
Dissecting the single-cell transcriptome network underlying esophagus non-malignant tissues and esophageal squamous cell carcinoma-EBioMedicine
IL-8/CXCR2 mediates tropism of human bone marrow-derived mesenchymal stem cells toward CD133+/CD44+ Colon cancer stem cells-Journal of Cellular Physiology (J Cell Physiol)
Co-delivery of autophagy inhibitor and gemcitabine using a pH-activatable core-shell nanobomb inhibits pancreatic cancer progression and metastasis-Theranostics
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